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chromium next gem single cell 3ʹ reagent kit v3 1 dual index  (10X Genomics)

 
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    10X Genomics chromium next gem single cell 3ʹ reagent kit v3 1 dual index
    Chromium Next Gem Single Cell 3ʹ Reagent Kit V3 1 Dual Index, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+3%CA%B9+gem+kit/cellranger/pm42185256-282-17-29
    Average 86 stars, based on 1 article reviews
    chromium next gem single cell 3ʹ reagent kit v3 1 dual index - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Encapsulation:

    Article Title: Bacteria hijack a meningeal neuroimmune axis to facilitate brain invasion.
    Article Snippet: Encapsulation of cells was performed in a Chromium Controller (10x Genomics) targeting 10,000 cells per sample. .. Following encapsulation and mRNA barcoding, cDNA was synthesized, isolated and amplified (11 cycles) using a Single Cell 3ʹ GEM kit (10x Genomics) and a SPRIselect reagent kit (Beckman Coulter). .. The quality of the amplified cDNA (concentration, size and purity) was verified using a High Sensitivity D5000 ScreenTape and 4200 TapeStation system (Agilent Technologies).

    Synthesized:

    Article Title: Bacteria hijack a meningeal neuroimmune axis to facilitate brain invasion.
    Article Snippet: Encapsulation of cells was performed in a Chromium Controller (10x Genomics) targeting 10,000 cells per sample. .. Following encapsulation and mRNA barcoding, cDNA was synthesized, isolated and amplified (11 cycles) using a Single Cell 3ʹ GEM kit (10x Genomics) and a SPRIselect reagent kit (Beckman Coulter). .. The quality of the amplified cDNA (concentration, size and purity) was verified using a High Sensitivity D5000 ScreenTape and 4200 TapeStation system (Agilent Technologies).

    Isolation:

    Article Title: Bacteria hijack a meningeal neuroimmune axis to facilitate brain invasion.
    Article Snippet: Encapsulation of cells was performed in a Chromium Controller (10x Genomics) targeting 10,000 cells per sample. .. Following encapsulation and mRNA barcoding, cDNA was synthesized, isolated and amplified (11 cycles) using a Single Cell 3ʹ GEM kit (10x Genomics) and a SPRIselect reagent kit (Beckman Coulter). .. The quality of the amplified cDNA (concentration, size and purity) was verified using a High Sensitivity D5000 ScreenTape and 4200 TapeStation system (Agilent Technologies).

    Amplification:

    Article Title: Bacteria hijack a meningeal neuroimmune axis to facilitate brain invasion.
    Article Snippet: Encapsulation of cells was performed in a Chromium Controller (10x Genomics) targeting 10,000 cells per sample. .. Following encapsulation and mRNA barcoding, cDNA was synthesized, isolated and amplified (11 cycles) using a Single Cell 3ʹ GEM kit (10x Genomics) and a SPRIselect reagent kit (Beckman Coulter). .. The quality of the amplified cDNA (concentration, size and purity) was verified using a High Sensitivity D5000 ScreenTape and 4200 TapeStation system (Agilent Technologies).

    Single Cell:

    Article Title: Bacteria hijack a meningeal neuroimmune axis to facilitate brain invasion.
    Article Snippet: Encapsulation of cells was performed in a Chromium Controller (10x Genomics) targeting 10,000 cells per sample. .. Following encapsulation and mRNA barcoding, cDNA was synthesized, isolated and amplified (11 cycles) using a Single Cell 3ʹ GEM kit (10x Genomics) and a SPRIselect reagent kit (Beckman Coulter). .. The quality of the amplified cDNA (concentration, size and purity) was verified using a High Sensitivity D5000 ScreenTape and 4200 TapeStation system (Agilent Technologies).



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    a Timeline and schematic of the experimental design. b Gating strategy for isolating tdTom+ cells from the live mesenchymal population for <t>single</t> <t>cell</t> RNA sequencing (scRNA-seq). c Quantification of sorted lineage-labeled cells in saline and experimental groups. d Integrative Uniform Manifold Approximation and Projection (UMAP) plot of lineage-labeled Fgf10 + cells isolated from Sal, Bleo d14, Bleo d30, and Bleo d60 showing different clusters. e Frequency of clusters in each condition. f Heatmap showing the top DEGs in each cluster. g RNA velocity analysis for individual timepoints. h Integrative RNA cell velocity with latent inferred time and the corresponding dynamical genes. i , j UMAP plots showing the expression patterns of indicated genes. k Dot plot showing co-expression of Adamts4 and indicated genes. Source data are provided as a file. In c n = 4 per group; each data point represents one biological replicate. Data are presented as mean ± SEM. Statistical analysis was performed using ordinary one-way ANOVA with Dunnett’s multiple comparisons test. * P < 0.05; *** P < 0.001; **** P < 0.0001. Adamts4 ADAM metallopeptidase with thrombospondin type 1 motif 4, AF1 Alveolar fibroblasts 1, AF2 Alveolar fibroblasts 2, Bleo Bleomycin, d.p.i. days post instillation, EpCAM Epithelial cell adhesion molecule, Fgf10 Fibroblast growth factor 10, Hhip Hedgehog interacting protein, i.p. Intraperitoneal injection, MyoFB Myofibroblasts, PeriFB Peribronchial fibroblasts, Pi16 Peptidase inhibitor 16, Sal Saline, Scube2 Signal peptide, CUB domain and EGF like domain containing protein 2, Tam Tamoxifen; tdTom: tdTomato.
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    a Timeline and schematic of the experimental design. b Gating strategy for isolating tdTom+ cells from the live mesenchymal population for <t>single</t> <t>cell</t> RNA sequencing (scRNA-seq). c Quantification of sorted lineage-labeled cells in saline and experimental groups. d Integrative Uniform Manifold Approximation and Projection (UMAP) plot of lineage-labeled Fgf10 + cells isolated from Sal, Bleo d14, Bleo d30, and Bleo d60 showing different clusters. e Frequency of clusters in each condition. f Heatmap showing the top DEGs in each cluster. g RNA velocity analysis for individual timepoints. h Integrative RNA cell velocity with latent inferred time and the corresponding dynamical genes. i , j UMAP plots showing the expression patterns of indicated genes. k Dot plot showing co-expression of Adamts4 and indicated genes. Source data are provided as a file. In c n = 4 per group; each data point represents one biological replicate. Data are presented as mean ± SEM. Statistical analysis was performed using ordinary one-way ANOVA with Dunnett’s multiple comparisons test. * P < 0.05; *** P < 0.001; **** P < 0.0001. Adamts4 ADAM metallopeptidase with thrombospondin type 1 motif 4, AF1 Alveolar fibroblasts 1, AF2 Alveolar fibroblasts 2, Bleo Bleomycin, d.p.i. days post instillation, EpCAM Epithelial cell adhesion molecule, Fgf10 Fibroblast growth factor 10, Hhip Hedgehog interacting protein, i.p. Intraperitoneal injection, MyoFB Myofibroblasts, PeriFB Peribronchial fibroblasts, Pi16 Peptidase inhibitor 16, Sal Saline, Scube2 Signal peptide, CUB domain and EGF like domain containing protein 2, Tam Tamoxifen; tdTom: tdTomato.
    Chromium Next Gem Single Cell 3ʹ Reagents Kit V3 1, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+3%CA%B9+gem+kit/cellranger/bio_rxiv__64898__2026__04__28__721483-262-9-18
    Average 86 stars, based on 1 article reviews
    chromium next gem single cell 3ʹ reagents kit v3 1 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    a Timeline and schematic of the experimental design. b Gating strategy for isolating tdTom+ cells from the live mesenchymal population for single cell RNA sequencing (scRNA-seq). c Quantification of sorted lineage-labeled cells in saline and experimental groups. d Integrative Uniform Manifold Approximation and Projection (UMAP) plot of lineage-labeled Fgf10 + cells isolated from Sal, Bleo d14, Bleo d30, and Bleo d60 showing different clusters. e Frequency of clusters in each condition. f Heatmap showing the top DEGs in each cluster. g RNA velocity analysis for individual timepoints. h Integrative RNA cell velocity with latent inferred time and the corresponding dynamical genes. i , j UMAP plots showing the expression patterns of indicated genes. k Dot plot showing co-expression of Adamts4 and indicated genes. Source data are provided as a file. In c n = 4 per group; each data point represents one biological replicate. Data are presented as mean ± SEM. Statistical analysis was performed using ordinary one-way ANOVA with Dunnett’s multiple comparisons test. * P < 0.05; *** P < 0.001; **** P < 0.0001. Adamts4 ADAM metallopeptidase with thrombospondin type 1 motif 4, AF1 Alveolar fibroblasts 1, AF2 Alveolar fibroblasts 2, Bleo Bleomycin, d.p.i. days post instillation, EpCAM Epithelial cell adhesion molecule, Fgf10 Fibroblast growth factor 10, Hhip Hedgehog interacting protein, i.p. Intraperitoneal injection, MyoFB Myofibroblasts, PeriFB Peribronchial fibroblasts, Pi16 Peptidase inhibitor 16, Sal Saline, Scube2 Signal peptide, CUB domain and EGF like domain containing protein 2, Tam Tamoxifen; tdTom: tdTomato.

    Journal: Nature Communications

    Article Title: Persistence of alveolar fibroblast-derived ADAMTS4+ cells in a preclinical model of delayed pulmonary fibrosis resolution

    doi: 10.1038/s41467-026-72419-3

    Figure Lengend Snippet: a Timeline and schematic of the experimental design. b Gating strategy for isolating tdTom+ cells from the live mesenchymal population for single cell RNA sequencing (scRNA-seq). c Quantification of sorted lineage-labeled cells in saline and experimental groups. d Integrative Uniform Manifold Approximation and Projection (UMAP) plot of lineage-labeled Fgf10 + cells isolated from Sal, Bleo d14, Bleo d30, and Bleo d60 showing different clusters. e Frequency of clusters in each condition. f Heatmap showing the top DEGs in each cluster. g RNA velocity analysis for individual timepoints. h Integrative RNA cell velocity with latent inferred time and the corresponding dynamical genes. i , j UMAP plots showing the expression patterns of indicated genes. k Dot plot showing co-expression of Adamts4 and indicated genes. Source data are provided as a file. In c n = 4 per group; each data point represents one biological replicate. Data are presented as mean ± SEM. Statistical analysis was performed using ordinary one-way ANOVA with Dunnett’s multiple comparisons test. * P < 0.05; *** P < 0.001; **** P < 0.0001. Adamts4 ADAM metallopeptidase with thrombospondin type 1 motif 4, AF1 Alveolar fibroblasts 1, AF2 Alveolar fibroblasts 2, Bleo Bleomycin, d.p.i. days post instillation, EpCAM Epithelial cell adhesion molecule, Fgf10 Fibroblast growth factor 10, Hhip Hedgehog interacting protein, i.p. Intraperitoneal injection, MyoFB Myofibroblasts, PeriFB Peribronchial fibroblasts, Pi16 Peptidase inhibitor 16, Sal Saline, Scube2 Signal peptide, CUB domain and EGF like domain containing protein 2, Tam Tamoxifen; tdTom: tdTomato.

    Article Snippet: Cells were flow-sorted, and libraries were prepared using the Chromium Next GEM Single-Cell 3ʹ Reagent Kit v3.1 from 10x Genomics.

    Techniques: Single Cell, RNA Sequencing, Labeling, Saline, Isolation, Expressing, Injection